Nucleic Acid Conversions

Conversion of Weight to Absolute Quantity (mol)

1 ľg of 1,000 bp DNA = 1.52 pmol = 9.1 x 1011 molecules
1 ľg of pUC18/19 DNA (2,686 bp) = 0.57 pmol = 3.4 x 1011 molecules
1 ľg of pBR322 DNA (4,361 bp) = 0.35 pmol = 2.1 x 1011 molecules
1 ľg of M13mp18/19 DNA (7,249 bp) = 0.21 pmol = 1.3 x 1011 molecules
1 ľg of ? DNA (48,502 bp) = 0.30 pmol = 1.8 x 1010 molecules

Conversion of Absolute Quantity (mol) to Weight

1 pmol of 1,000 bp DNA = 0.66 ľg
1 pmol of pUC18/19 DNA (2,686 bp) = 1.77 ľg
1 pmol of pBR322 DNA (4,361 bp) = 2.88 ľg
1 pmol of M13mp18/19 DNA (7,249 bp) = 4.78 ľg
1 pmol of DNA (48,502 bp) = 32.01 ľg

Molecular Weights for Nucleotides

Compound Molecular weight
AMP 347.2
CMP 323.2
GMP 363.2
UMP 324.2
dAMP 331.2
dCMP 307.2
dGMP 347.2
dTMP 322.2

Molecular Weight of DNA Fragments

500 bp dsDNA = 325,000 Dalton
500 nt (Nucleotide) ssDNA = 162,500 Dalton
1 kb dsDNA = 660,000 Dalton
1 kb ssDNA = 330,000 Dalton
1 kb ssRNA = 340,000 Dalton
1 MDa of dsDNA = 1.52 kb

The average molecular weight of dNMP is 325 Dalton.
The average molecular weight of DNA base pair is 650 Dalton.

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